Characterization of Genomic and Coding Sequence Variation in the SKI2 Locus Following Whole Genome Duplication : identification and characterization of coding sequence and stop codon variation using MAFFT and comparative alignment

Sammanfattning

This study investigated amino acid mutations in the SKI2 protein between diploid and tetraploid Mimulus plants following whole genome duplication (WGD) (See figure 1). By comparing protein sequences using multiple sequence alignment and analyzing predicted AlphaFold structures. 12 tetraploid-specific amino acid changes were found. Most changes were conservative and located in less functional regions, but three substitutions (S584Y, L918Q, and T1331M) stood out as strong candidates for potential functional changes.