Histopathological assessment of canine lymphoma, with a focus on the inflammatory microenvironment and serglycin levels in affected lymph nodes

Sammanfattning

Research has shown that inflammation plays a significant role in the microenvironments of several cancer types, contributing to the pathogenesis and affecting prognosis. This study proposed an investigation of the microenvironment in canine lymphoma, where much of this is still unknown. This study aimed to analyze the degree of inflammation, serglycin expression, and mitotic rate in formalin-fixed and paraffin-embedded lymphoma tissue from canine lymph nodes. The study utilized 13 unclassified lymphomas, classified as B or T as part of the study, along with eight controls, reactive lymph nodes with typical architecture. The results could not provide evidence that there is inflammation in canine lymph nodes with lymphoma, however, it could neither rule out that there is an inflammation that primarily consists of lymphocytes and macrophages, (with low involvement of other inflammatory cells) since macro-phages and lymphocytes were abundant in both cases and reactive controls. Of the lymphomas, seven were classified as B-type, three as T-type, and three could neither be classified as B, nor T. Serglycin expression in the lymphoma tissue was significantly lower than in the control tissue. Initial insight into the levels of serglycin expression in canine lymphoma is provided by this study, and further investigation is required to understand its significance. The study's findings could help ,guide future research on canine lymphoma. Follow-up studies could include the classification and distribution of different subtypes of lymphocytes and macrophages, more cases, and lymphoma tissues from other organs. They could also include more advanced methods such as single-cell RNA sequencing, which can classify all cells in a tissue on transcriptome level.