Live-cell Microscopy Imaging of Drug-treated K562 and L428 Cancer Cells

Sammanfattning

The purpose of this project was to develop and optimise a protocol for live-cell imaging with the Nikon Eclipse Ti2-E Live-cell Fluorescence Imaging microscope by using two types of blood cancers cell lines, K562 and L428. The clearest visualisation for dual staining of the K562 was achieved with the concentrations 0.05 μg/ml of Hoechst dye and 2.0 μg/ml for ViaFluor dye. The clearest images of L428 were obtained using 0.005 μg/ml of Hoechst and 1.0 μg/ml for ViaFluor. The morphological response of Doxorubicin and Vinblastine treatment were then monitored using the obtained protocol and visualised with the two dyes and examined at 4 different time points. Clear morphological changes as a result of treatment could be observed as well as an increase in uptake of dye.