Phenol-Protein Solution Stability

Sammanfattning

Phenol is a commonly used preservative in pharmaceutical protein solutions. It is compatible with most proteins but can cause aggregation and denaturation at higher concentrations or in more sensitive proteins. By titrating phenol in 12 different protein solutions with PS80 (Lysozyme, β -lactoglobulin, SOBI Lipase, GA-Z, Palifermin and Trastuzumab, all with and without NaCl) the phenol concentration at which the protein solution starts to be negatively affected has been determined. The impact of salt on the phenol sensitivity of the solutions has also been studied and DLS and SAXS tests have been made. The effects of PS80 has also been studied to determine if the protein or the surfactant is more sensitive to phenol.