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Structure and function of CpI andTamHydS Hydrogenase

Master-uppsats

Åkerfeldt, Viktor

Uppsala universitet/Biokemi

Publicerad: 2025

Språk: Engelska

Nyckelord

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⌕Hydrogen⌕FTIR-spectroscopy⌕CpI⌕Tamhyds⌕hydrogenase⌕CryoEM⌕Clostridium pasteurianum⌕C. pasteurianum

Sammanfattning

In this master thesis, the CpI hydrogenase was produced and purified. A structure was resolved using cryoEM, showing a similar structure to its X-ray crystallography counterpart, though with lower resolution. The first experimental 2D images of oxygen-sensing TamHydS hydrogenase were obtained with cryoEM, but lack the fidelity to reconstruct a 3D model. The CpI function was studied with FTIR-spectroscopy, and its redox state during catalysis was determined.

Information

Författare
Åkerfeldt, Viktor
Lärosäte / institution
Uppsala universitet/Biokemi
Publiceringsdatum
2025
Uppsatstyp
Master-uppsats
Språk
Engelska
Nyckelord
⌕Hydrogen⌕FTIR-spectroscopy⌕CpI⌕Tamhyds⌕hydrogenase⌕CryoEM⌕Clostridium pasteurianum⌕C. pasteurianum

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